Electronic PCR (e-PCR)
What is e-PCR?
e-PCR identifies sequence tagged sites(STSs)within DNA sequences. Using e-PCR, you can search for sub-sequences that closely match the PCR primers and have the correct order, orientation, and spacing.
What's New?
Improved search sensitivity
You can use multiple discontigous words instead of a single exact word. Each of this word has groups of significant positions seperated by 'wildcard' positions. It is not required that these positions match. Also, it is now possible to allow gaps in the primer alignments. A fuzzy matching strategy reduces the likelihood that a true STS will be missed due to mismatches.
Reverse searching
Searching the human genome sequence and other large genomes is now possible. The new version of e-PCR provides a search mode using a query sequence against a sequence database.
Publications
Sequence mapping by electronic PCR
Schuler GD. Genome Res. 1997 May;7(5):541-50.
A web server for performing electronic PCR
Rotmistrovsky K, Jang W, Schuler GD. Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W108-12.