Nathan Grubaugh
@NathanGrubaugh
Assistant Professor @YaleEMD. Studies 
transmission, evolution, and emergence. Will sequence for 
(and funding).
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1/n An overdue thread on
#SalivaDirect... No doubt many of you heard of it, either on this platform, or on the news. It generated a lot of buzz in the media, and not all of it is true. Lets walk through these, and how to get it set up in your lab.https://covidtrackerct.com/about-salivadirect/ …Show this threadThanks. Twitter will use this to make your timeline better. UndoUndo -
Nathan Grubaugh Retweeted
Back in March, we started posting updates about SARS-CoV-2 spread in Connecticut. The team learned a few things along the way, so the team decided to collect our goals and lessons learned in a perspective now in @PLOSBio: https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3000869 …https://twitter.com/PLOSBiology/status/1297934193201209344 …
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Nathan Grubaugh Retweeted
Happy to present our new preprint on a new mosquito mark-release-recapture method we have developed with
@faimanr,@friend2inverts,@tovilehmann,@ZanaLamissa and others not on twitter 1/nhttps://twitter.com/faimanr/status/1297932378510303232 …Show this threadThanks. Twitter will use this to make your timeline better. UndoUndo -
Nathan Grubaugh Retweeted
Communicating the
#COVID19 pandemic in real-time.@ChaneyKalinich@NathanGrubaugh@AndersonBrito_ &co propose that presenting SARS-CoV-2 phylogenetic analyses rapidly, and tailored to non-experts, can improve public health communication#PLOSBiology https://plos.io/3hoo5x0 pic.twitter.com/gHybVTqP3m
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Nathan Grubaugh Retweeted
Rhode Island announces first EEE detection of 2020https://www.ri.gov/press/view/39152 …
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Nathan Grubaugh Retweeted
THREAD: For the last 6 months, FDA’s device center worked effectively with labs to advance hundreds of tests for Covid. A new HHS policy that extricates FDA from this work - and goes further, by removing any FDA role over any lab developed test - could put this work at risk. 1/x
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What does this mean? Will labs not have to apply for an EUA to use a new SARS-CoV-2 test? Could be a nightmare for ensuring testing standards, but would make my job a heck of a lot easer...
https://www.fiercebiotech.com/medtech/trump-administration-revokes-fda-authority-over-lab-developed-tests-including-some-covid-19 …Thanks. Twitter will use this to make your timeline better. UndoUndo -
Nathan Grubaugh Retweeted
Pleased to announce that
@LCoffeyLab and I have organized a virtual Virus Evolution seminar series for the fall semester. Mondays: 3-4 pm CST Registration: https://umn.zoom.us/meeting/register/tJMrceuoqDktGNe5TykqgNlZHROhQYle3O85 … Please RTpic.twitter.com/fWrhOpm1Va
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Nathan Grubaugh Retweeted
August 20, 2020--Mosquitoes in State Test Positive for Equine Encephalitis Virushttps://www.primepublishers.com/towntimesnews/news/community_news/mosquitoes-in-state-test-positive-for-equine-encephalitis-virus/article_51697a30-e258-11ea-9e23-2bb3faa5e6f7.html …
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Nathan Grubaugh Retweeted
Ladies and germs, it’s time for the thread you didn’t realize you were waiting for: my ride-along narration of
@aewatkins6 doing a SalivaDirect run! (1/??)https://twitter.com/nathangrubaugh/status/1295813821798535168 …Show this threadThanks. Twitter will use this to make your timeline better. UndoUndo -
Nathan Grubaugh Retweeted
Work from my time in Alex Bukreyev's lab describing species-specific adaptive plasticity of
#Ebola virus in#bat and human cell lines. Importantly, we add to the growing body of evidence implicating ADAR-mediated editing of EBOV genomes during infectionhttps://www.cell.com/cell-reports/fulltext/S2211-1247(20)31013-5 …Show this threadThanks. Twitter will use this to make your timeline better. UndoUndo -
19/n If you are a postdoc and would like help develop the next generation of SalivaDirect, please let us know!https://covidtrackerct.com/jobs/
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18/n If you have questions, please direct them to salivadirect@gmail.com and not my DMs or my personal email. Thank you!
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17/n What's next? Pooling. By combining saliva from many people and testing it all at once, we can increase the number of samples that can be tested per day and reduce the amount of each reagent used per person.
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16/n What's next? Rapid detection. We are working on approaches to replace the PCR step with either LAMP or RPA. PCR will still have a role, but a simple rapid test has the ability to be point-of-care.
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15/n What's next? Automation. We are working with several institutions to validate robotic systems to automate the sample processing and/or PCR. Once these are validated, we'll add the protocols to our EUA for others to use.
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14/n Once we are set, we will prioritize authorizing labs that will test schools first.
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13/n We are currently working with Yale and FDA legal teams to draft the conditions of the authorization and to formalize regulatory oversight of the process. As this is the first time that the FDA has issued a protocol EUA, it will take us a bit to get the documents approved.
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13/n How to become an authorized lab to use SalivaDirect? If you represent a high complexity CLIA certified lab within the United States and would like to become authorized, please follow the steps listed on our website:https://covidtrackerct.com/about-salivadirect/ …
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12/n Also, we are currently not able to offer testing to the public, although we are working to develop testing partnerships that may be of use to you or your organization.
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11/n How will SalivaDirect be offered? Only by authorized CLIA certified labs. At this moment we cannot help you to find or set up a lab to run SalivaDirect, as we are focused on authorizing labs that are currently CLIA certified.
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